Fab Rat IgG (H&L) Antibody

SKU: orb348501

Description

Fab Rat IgG (H&L) antibody

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:5,000 - 1:85,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Rat
Application Notes	Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG Fab
Immunogen	Rat IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Fab Anti-Rat IgG Antibody, Rabbit Fab Fragment Anti-Rat IgG Antibody

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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