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KI-67 Antibody
SKU: orb1735092
Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:50 – 1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide /Ki-67 |
| Target | KI-67 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1735092 anti-Ki-67 antibody at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 antibody with orb1735092 at 1/100.
This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1735092 overnight at 4°C. Lysates at 35 µg per lane. Anti Ki-67 antibody - at 1/500 dilution.
Quick Database Links
Gene Symbol
KI-67
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
KI-67 Antibody (orb1735092)
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